The Adisave procedure uses an entirely closed system, meaning once your adipose tissue is removed in the operating room, it is never exposed to air again.
When your fat tissue arrives at our facility, we process it in our GMP (Good Manufacturing Practices) certified clean room (ISO 5 inside of ISO 7). A GMP clean room represents the highest standard of contaminant control, a level of clean room technology that few manufacturing plants, research centers and even hospitals can match.
Once your fat tissue arrives in our facility it is thoroughly washed to remove any blood or residual substances such as local anesthetic.
We digest the fat tissue with collagenase for 45 minutes.
We separate the stem cells from the fat tissue and neutralize it with your serum.
We isolate the stem cells from the surrounding material in our GMP machine called a SEPAX2.
We perform a validation of the stem cells, checking both the number of stem cells and their viability (the number of live healthy cells). We check for apoptosis (the process of programmed cell death). On average, we maintain a high ratio of viable healthy stem cells - approximately 94%.
We mix the stem cells with a cryo-protectant and your own serum, and freeze them in a computer-controlled rate freezer.