ADSC-SVF-002 is a novel cellular therapy product – its use has not yet been investigated in humans. It is an autologous, minimally manipulated, adipose derived stem cell (ADSC)-containing stromal vascular fraction (SVF) obtained from subcutaneous fat harvested by liposuction from patients. As a cellular therapy product, ADSC-SVF-002 (fresh or cryopreserved) is intended to be injected locally, with or without unprocessed autologous fat (fresh or cryopreserved), into soft tissue or damaged tissue.
This pilot study is to investigate the use of ADSC-SVF-002 for the treatment of wounds and soft tissue defects.
Health Canada issued a “No Objection Letter” on September 5, 2013 and Integreview IRB granted Research Ethical Board approval on September 17, 2013.
Adisave has focused on finding optimal methods for harvesting, separation, extraction, and cryopreservation of Adipose Derived Stem Cells (ADSCs) from lipoaspirates. The main focus of this abstract presentation is to demonstrate how enzymatic and mechanical separations affect characteristics of hADSCs. Adipose derived stem cells hold great promise in cellular therapy.
They may surpass any other source of adult mesenchymal stem cells by their particular characteristics such as abundance, accessibility, homing and immuno-privileges. While the ultimate goal is to use these characteristics as a unique opportunity to change the paradigm of medicine, care must be taken to avoid any manipulations that introduce irreversible changes in hADSCs characteristics.
In this study for the first time we comparatively analyze viability, sterility, cell surface molecules, colony forming ability, differentiation potentials and genetic stability of hADSCs. We discuss the impact of liposuction techniques on the outcomes of fat grafting. Autologous fat grafting has been used to treat soft tissue anomalies since 1893 with variable results. The effectiveness of fat grafting may lie in the transfer of hADSCs with the fat that has the regenerative capacity to exit their quiescent state and enter active cell cycle in response to hypoxic conditions generated by the surrounding dying fat. Our study shows that the hADSCs separated from lipoaspirates harvested by water-jet liposuction technique lose properties of stemness and therefore might affect outcomes of fat grafting procedures.
Cryopreservation of adult stem cells has been discussed frequently in the literature. It has been demonstrated that storage temperature, freezing rate, cell concentration, and cryoprotectant variables play major roles in post-thaw recovery, viability and functionality of hADSCs. In this abstract we discuss how freezing rate, cell concentration and choice of cryoprotectants affect recovery, viability and functionality of hADSCs for the first time, in a completely autologous environment. We describe a method for autologous cryopreservation of hADSCs that facilitates their clinical use. The hADSCs functional status and morphologic characteristics were not changed by the autologous cryopreservation procedure described by our group. We also demonstrated that hADSCs are relatively resistant when exposed to Dimethyl Sulfoxide (DMSO).
Stem cells are at the center of expectations for regenerative medicine. We may be able to develop new and more potent regenerative therapeutics strategies if we can decipher the extracellular-intracellular mechanisms responsible for developmental patterning in the embryo and adult. Mechanical forces can switch cell fate between growth, differentiation and death. It has been shown that most mechanical signalling pathways are induced and mediated by integrin receptors and integrin linked kinases. Regenerative capacity of a tissue relies on the quiescent stem cells resident within the tissue. Different approaches have been introduced to utilize this capacity from different tissues. Adipose derived stem cells are a heterogeneous population of cells with multi-potent regenerative capacity. In this study we demonstrated how different methods of separation of cells from lipoaspirate could greatly affect their regenerative capacity. The field is still young and it seems likely that cellular therapies using autologous stem cells will be effective for certain conditions. Utilization of the regenerative capacity of adult stem cells requires diligent confirmation of all processes if the ultimate goal is to preserve their full regenerative capacity for therapeutic strategies.